6. Enzymes and Enzyme Kinetics

Which of the following rate constants is negligible for the initial velocity (V ₀) of an enzyme-catalyzed reaction?

Calculate the reaction rate for the following simple reaction if k = 1.3 x 10 ^-1 M^-1s^-1, initial [A] = 4.0 x 10^-3 M, and the initial [B] = 6.0 x 10 ^-3 M (for simple reactions, assume coefficients are reaction orders):

A + B → 2C

Write out the rate law equations for each association/dissociation indicated below.

a) Rate law for ES dissociation into E + P: V = ______________

b) Rate law for E + S association: V = ______________

c) Rate law for ES dissociation back into E + S: V = ______________

In a typical enzyme-catalyzed reaction, when & why is the rate constant k  _-2 negligible?