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Multiple Choice
DNA ligase functions in which of the following processes?
A
Joining Okazaki fragments during DNA replication
B
Unwinding the DNA helix during replication
C
Removing RNA primers from the lagging strand
D
Synthesizing RNA primers for DNA replication
Verified step by step guidance
1
Understand the role of DNA ligase in DNA replication. DNA ligase is an enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
Recognize the context of DNA replication, particularly the synthesis of the lagging strand, which involves the creation of Okazaki fragments.
Identify the process of joining Okazaki fragments. During DNA replication, the lagging strand is synthesized in short segments known as Okazaki fragments. DNA ligase is responsible for joining these fragments to create a continuous DNA strand.
Differentiate the roles of other enzymes involved in DNA replication. For example, helicase unwinds the DNA helix, and RNA primers are synthesized by primase, not DNA ligase.
Conclude that DNA ligase's specific function in DNA replication is to join Okazaki fragments, making it distinct from the other processes listed.