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Multiple Choice
Which enzyme seals Okazaki fragments together during DNA replication?
A
Primase
B
DNA polymerase
C
Helicase
D
DNA ligase
Verified step by step guidance
1
Understand the process of DNA replication: During DNA replication, the DNA double helix is unwound, and each strand serves as a template for the synthesis of a new complementary strand.
Identify the role of Okazaki fragments: On the lagging strand, DNA is synthesized in short segments known as Okazaki fragments because DNA polymerase can only add nucleotides in the 5' to 3' direction.
Recognize the need for joining Okazaki fragments: These fragments need to be joined together to form a continuous DNA strand.
Learn about the function of DNA ligase: DNA ligase is the enzyme responsible for sealing the nicks between Okazaki fragments, creating a continuous DNA strand by forming phosphodiester bonds.
Differentiate between the enzymes: Primase synthesizes RNA primers, DNA polymerase extends the DNA strand, and helicase unwinds the DNA helix. DNA ligase specifically seals the Okazaki fragments.