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Multiple Choice
Which components are needed for the expression phase of the CRISPR-Cas system?
A
DNA polymerase and helicase
B
Ribosomes and tRNA
C
RNA polymerase and mRNA
D
crRNA and tracrRNA
Verified step by step guidance
1
Understand the CRISPR-Cas system: It is a bacterial immune system that provides resistance against foreign genetic elements such as plasmids and phages. It involves two main phases: adaptation and expression.
Identify the expression phase: In this phase, the CRISPR array is transcribed into a long precursor CRISPR RNA (pre-crRNA), which is then processed into individual CRISPR RNAs (crRNAs).
Recognize the role of crRNA: crRNA guides the Cas proteins to the target DNA by base pairing with the complementary sequence in the foreign DNA.
Understand the function of tracrRNA: tracrRNA is required for the maturation of crRNA. It forms a complex with crRNA and Cas9, facilitating the cleavage of target DNA.
Conclude the components needed: For the expression phase of the CRISPR-Cas system, crRNA and tracrRNA are essential as they work together to guide the Cas proteins to the target DNA for cleavage.