26. Prokaryotes

Cyanide (C ≡ N⁻) blocks complex IV of the electron transport chain. Suggest a hypothesis for what happens to the ETC when complex IV stops working. Your hypothesis should explain why cyanide poisoning in humans is fatal.

Suppose that you've been hired by a firm interested in using bacteria to clean up organic solvents found in toxic waste dumps. Your new employer is particularly interested in finding cells that are capable of breaking a molecule called benzene into less-toxic compounds. Where would you go to look for bacteria that can metabolize benzene as an energy or carbon source? How would you design an enrichment culture capable of isolating benzene-metabolizing species?

Which of the following experiments could test the hypothesis that bacteria cause ulcers in humans? (Assume each experiment includes a control group.) Explain what evidence would be provided by the results of the experiment.

a. Identify the microbes found in the stomachs of ulcer patients.

b. Treat a group of ulcer patients with antibiotics.

c. Place a group of ulcer patients on a strict low-acid diet.

d. Obtain stomach fluid from ulcer patents and feed it to mice.

Probiotics, foods and supplements that contain living microorganisms, are thought to cure problems of the digestive tract by restoring the natural balance of its microbial community. Sales of these products total billions of dollars a year. Explore the topic of probiotics and evaluate the scientific evidence for their beneficial effects. A good starting point is the website of the U.S. Food and Drug Administration, which regulates advertising claims of health benefits of dietary supplements. U.S. Food and Drug Administration website, www.fda.gov/food/dietary-supplements.

Researchers examined the relationship between gut microbiomes and depression. To do so, they collected fecal samples from people with depression and also a control group of individuals with no signs of depression. They then performed a fecal microbiota transfer (FMT) by adding the samples to rats that had no gut microbiota and examined behaviors associated with depression and anxiety as well as species of bacteria that ended up growing in the rats’ guts. The results are presented below. Graph (a) shows the rats’ interest in a pleasurable experience (drinking sugar water). Graph (b) shows the amount of time rats spent out in the open versus along the edge of an area (a sign of anxiety). Graph (c) shows the number of species observed in rats after FMT.

What conclusion can be drawn regarding the relationship between microbiome diversity and mental health in rats?

Researchers examined the relationship between gut microbiomes and depression. To do so, they collected fecal samples from people with depression and also a control group of individuals with no signs of depression. They then performed a fecal microbiota transfer (FMT) by adding the samples to rats that had no gut microbiota and examined behaviors associated with depression and anxiety as well as species of bacteria that ended up growing in the rats’ guts. The results are presented below. Graph (a) shows the rats’ interest in a pleasurable experience (drinking sugar water). Graph (b) shows the amount of time rats spent out in the open versus along the edge of an area (a sign of anxiety). Graph (c) shows the number of species observed in rats after FMT.

What conclusions can be drawn regarding the impact of FMT from depressed individuals on behaviors associated with depression and anxiety in rats?

Researchers examined the relationship between gut microbiomes and depression. To do so, they collected fecal samples from people with depression and also a control group of individuals with no signs of depression. They then performed a fecal microbiota transfer (FMT) by adding the samples to rats that had no gut microbiota and examined behaviors associated with depression and anxiety as well as species of bacteria that ended up growing in the rats’ guts. The results are presented below. Graph (a) shows the rats’ interest in a pleasurable experience (drinking sugar water). Graph (b) shows the amount of time rats spent out in the open versus along the edge of an area (a sign of anxiety). Graph (c) shows the number of species observed in rats after FMT.

Probiotics are often marketed as a food or supplement that can help promote a healthy gut. How might a healthy gut microbiome help improve mental health?

Researchers examined the relationship between gut microbiomes and depression. To do so, they collected fecal samples from people with depression and also a control group of individuals with no signs of depression. They then performed a fecal microbiota transfer (FMT) by adding the samples to rats that had no gut microbiota and examined behaviors associated with depression and anxiety as well as species of bacteria that ended up growing in the rats’ guts. The results are presented below. Graph (a) shows the rats’ interest in a pleasurable experience (drinking sugar water). Graph (b) shows the amount of time rats spent out in the open versus along the edge of an area (a sign of anxiety). Graph (c) shows the number of species observed in rats after FMT.

Why was it important for the rats used in the study to be free of gut microbiota to begin with?

Researchers examined the relationship between gut microbiomes and depression. To do so, they collected fecal samples from people with depression and also a control group of individuals with no signs of depression. They then performed a fecal microbiota transfer (FMT) by adding the samples to rats that had no gut microbiota and examined behaviors associated with depression and anxiety as well as species of bacteria that ended up growing in the rats’ guts. The results are presented below. Graph (a) shows the rats’ interest in a pleasurable experience (drinking sugar water). Graph (b) shows the amount of time rats spent out in the open versus along the edge of an area (a sign of anxiety). Graph (c) shows the number of species observed in rats after FMT.

Hypothesize how bacteria living in a person’s gut might influence events occurring in the brain.

Which of the following describes a bacterium that lives in the human intestine and causes disease?

a. Commensalist

b. Decomposer

c. Gut mutualist

d. Symbiotic pathogen

The prefix photo- indicates that an organism will make use of _______ for energy purposes.

Biologists can divide living organisms into two groups:autotrophs and heterotrophs, which differ in _________.

Organisms that use CO₂ as their source of carbon are called

Chemoheterotrophs: