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Multiple Choice
Which of the following is NOT involved in the unwinding of DNA during replication?
A
Topoisomerase
B
Single-strand binding proteins
C
Helicase
D
DNA ligase
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Verified step by step guidance
1
Step 1: Understand the role of each protein listed in the context of DNA replication, specifically focusing on the unwinding process.
Step 2: Recall that Helicase is the enzyme responsible for breaking hydrogen bonds between DNA strands, effectively unwinding the double helix.
Step 3: Recognize that Single-strand binding proteins stabilize the separated DNA strands to prevent them from re-annealing during replication.
Step 4: Know that Topoisomerase relieves the torsional strain ahead of the replication fork by cutting and rejoining DNA strands, facilitating unwinding.
Step 5: Identify that DNA ligase is involved later in replication, specifically in joining Okazaki fragments on the lagging strand, and does not participate in the unwinding process.