Multiple Choice
Which enzyme is responsible for joining DNA fragments during DNA repair?
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17. Mutation, Repair, and Recombination
DNA Repair
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Which protein joins together the Okazaki fragments of DNA in the lagging strand during DNA replication?
A
Primase
B
DNA polymerase I
C
DNA ligase
D
Helicase
Verified step by step guidance1
Understand the process of DNA replication, focusing on the lagging strand where DNA is synthesized discontinuously in short segments called Okazaki fragments.
Recall the role of each enzyme listed: Primase synthesizes RNA primers, DNA polymerase I removes RNA primers and replaces them with DNA, Helicase unwinds the DNA double helix.
Identify that Okazaki fragments are initially separate pieces of DNA that need to be joined to form a continuous strand.
Recognize that the enzyme responsible for joining these fragments by forming phosphodiester bonds between adjacent DNA fragments is DNA ligase.
Conclude that DNA ligase is the protein that joins Okazaki fragments together during lagging strand synthesis.
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